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Anti-INSL3 antibody [EPR14834(2)] - BSA and Azide free (ab251284)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14834(2)] to INSL3 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-INSL3 antibody [EPR14834(2)] - BSA and Azide free
    See all INSL3 primary antibodies

  • Description

    Rabbit monoclonal [EPR14834(2)] to INSL3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251284 is the carrier-free version of ab199536. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251284 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-INSL3 antibody [EPR14834(2)] - BSA and Azide free (ab251284)
    Western blot - Anti-INSL3 antibody [EPR14834(2)] - BSA and Azide free (ab251284)
    All lanes : Anti-INSL3 antibody [EPR14834(2)] (ab199536) at 1/5000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Human testis lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal kidney lysate
    Lane 5 : Human fetal spleen lysate
    Lane 6 : NCCIT (Human pluripotent embryonal carcinoma) whole cell lysate
    Lane 7 : A431 (Human epidermoid carcinoma) whole cell lysate
    Lane 8 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 3 minutes


    This data was developed using ab199536, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    INSL3 is expressed in prenatal and postnatal Leydig cells. Human fetal heart, kidney, spleen, NCCIT, A431, and Jurkat cells represent negative controls in this experiment.

  • Immunoprecipitation - Anti-INSL3 antibody [EPR14834(2)] - BSA and Azide free (ab251284)
    Immunoprecipitation - Anti-INSL3 antibody [EPR14834(2)] - BSA and Azide free (ab251284)
    This data was developed using ab199536, the same antibody clone in a different buffer formulation.INSL3 was immunoprecipitated from 1mg of Human testis whole cell lysate with ab199536 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199536 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Human testis whole cell lysate 10 µg (Input). Lane 2: ab199536 IP in Human testis whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199536 in Human testis whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  • Anti-INSL3 antibody [EPR14834(2)] - BSA and Azide free (ab251284)
    Anti-INSL3 antibody [EPR14834(2)] - BSA and Azide free (ab251284)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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