Anti-SH2D1A/SAP antibody [EPR3168] - BSA and Azide free (ab247769)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3168] to SH2D1A/SAP - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SH2D1A/SAP antibody [EPR3168] - BSA and Azide free
See all SH2D1A/SAP primary antibodies -
Description
Rabbit monoclonal [EPR3168] to SH2D1A/SAP - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247769 is the carrier-free version of ab109120. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247769 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3168 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/2000 dilution (purified)
Lane 1 : PC-12 whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?This data was developed using ab109120, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/2000 dilution (purified)
Lane 1 : MOLT-4 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : K562 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?This data was developed using ab109120, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/1000 dilution (unpurified)
Lane 1 : MOLT-4 cell line lysate
Lane 2 : Jurkat cell line lysate
Lane 3 : K562 cell line lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 14 kDaThis data was developed using ab109120, the same antibody clone in a different buffer formulation.
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