Human Anti-Respiratory syncytial virus IgA ELISA Kit (RSV) (ab108764)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
-
Product name
Human Anti-Respiratory syncytial virus IgA ELISA Kit (RSV) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Pos.Serum 24 4.9% Inter-assay Sample n Mean SD CV% Pos.Serum 12 5.2% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Respiratory syncytial virus (RSV) IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Respiratory syncytial virus in Human serum and plasma.
A 96-well plate has been precoated with Respiratory syncytial virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Respiratory syncytial virus -specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Respiratory syncytial virus IgA sample captured in plate.
-
Platform
Microplate
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Anti-Respiratory syncytial virus (IgA) Coated Microplate (12 x 8 wells) 1 unit Foil cover 1 unit IgA Sample Diluent yellow buffer, white cap 1 x 100ml Respiratory syncytial virus anti-IgA HRP Conjugate violet solution, black cap 1 x 20ml Respiratory syncytial virus IgA Cut-off Control yellow solution, green cap 1 x 3ml Respiratory syncytial virus IgA Negative Control yellow solution, blue cap 1 x 2ml Respiratory syncytial virus IgA Positive Control yellow solution, red cap 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Images
-
Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
