Anti-iNOS antibody [EP16634] - BSA and Azide free (ab251367)
![Anti-iNOS antibody [EP16634] - BSA and Azide free (ab251367)](https://www.abcam.com/ps/products/251/ab251367/Images/ab251367-405946-anti-inos-antibody-ep16634-bsa-and-azide-free-western-blot.jpg "Anti-iNOS antibody [EP16634] - BSA and Azide free (ab251367)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP16634] to iNOS - BSA and Azide free
- Suitable for: WB, ELISA
- Reacts with: Mouse, Human
Overview
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Product name
Anti-iNOS antibody [EP16634] - BSA and Azide free
See all iNOS primary antibodies -
Description
Rabbit monoclonal [EP16634] to iNOS - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251367 is the carrier-free version of ab202417. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251367 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EP16634 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-iNOS antibody [EP16634] - BSA and Azide free (ab251367)All lanes : Anti-iNOS antibody [EP16634] (ab202417) at 1/10000 dilution
Lane 1 : Untreated RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) treated with 0.1 mg/ml LPS for 6 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 131 kDa
Observed band size: 131 kDa
Exposure time: 3 minutesThis data was developed using ab202417, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![ELISA - Anti-iNOS antibody [EP16634] - BSA and Azide free (ab251367)](https://www.abcam.com/ps/products/251/ab251367/Images/ab251367-4-anti-inos-antibody-ep16634-elisa-house-mouse-inos2.jpg)
ELISA - Anti-iNOS antibody [EP16634] - BSA and Azide free (ab251367)This data was developed using ab202417, the same antibody clone in a different buffer formulation.
ELISA analysis of House mouse iNos2 recombinant protein at 1000 ng/mL with ab202417. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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![Western blot - Anti-iNOS antibody [EP16634] - BSA and Azide free (ab251367)](https://www.abcam.com/ps/products/251/ab251367/Images/ab251367-399183-anti-inos-antibody-ep16634-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-iNOS antibody [EP16634] - BSA and Azide free (ab251367)Anti-iNOS antibody [EP16634] (ab202417) at 1/1000 dilution + Human fetal brain lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 131 kDa
Observed band size: 131 kDa
Exposure time: 3 minutesThis data was developed using ab202417, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-iNOS antibody [EP16634] - BSA and Azide free (ab251367)](https://www.abcam.com/ps/products/251/ab251367/Images/ab251367-3-benefits-of-recombinant-antibodies.png)
Anti-iNOS antibody [EP16634] - BSA and Azide free (ab251367)