Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] - BSA and Azide free (ab264075)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22892-2] to IL2 Receptor beta/p75 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] - BSA and Azide free
    See all IL2 Receptor beta/p75 primary antibodies

  • Description

    Rabbit monoclonal [EPR22892-2] to IL2 Receptor beta/p75 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Human PBMC's;Flow Cyt: Human PBMC's.

  • General notes

    ab264075 is the carrier-free version of ab263906. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab264075 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] - BSA and Azide free (ab264075)
    Immunocytochemistry/ Immunofluorescence - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] - BSA and Azide free (ab264075)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized None cells labelling IL2 Receptor beta/p75 with ab263906 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in a subset of Human PBMC cells. is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263906).

  • Flow Cytometry - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] - BSA and Azide free (ab264075)
    Flow Cytometry - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] - BSA and Azide free (ab264075)

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL2 Receptor beta/p75 with ab263906 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) isotype control (left) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab263906 (Right). Then stained with anti-CD56 conjugated to BV421. IL2 Receptor beta/CD122 is constitutively expressed primarily by NK cells (PMID: 24055329). Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263906).

  • Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] - BSA and Azide free (ab264075)
    Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] - BSA and Azide free (ab264075)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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