Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-6 antibody [EPR22565-204] - BSA and Azide free (ab256355)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22565-204] to IL-6 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-IL-6 antibody [EPR22565-204] - BSA and Azide free
    See all IL-6 primary antibodies

  • Description

    Rabbit monoclonal [EPR22565-204] to IL-6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HUVEC treated with LPS then BFA (Brefeldin A) whole cell lysate. ICC/IF: HUVEC treated with LPS then BFA (Brefeldin A) cells. Flow Cyt: HUVEC treated with LPS then BFA (Brefeldin A) cells. IP: HUVEC treated with LPS then BFA (Brefeldin A) whole cell lysate.

  • General notes

    Ab256355 is the carrier-free version of ab233551. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256355 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-6 antibody [EPR22565-204] - BSA and Azide free (ab256355)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-6 antibody [EPR22565-204] - BSA and Azide free (ab256355)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labeling IL-6 with ab233551 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in HUVEC cells treated with lipopolysaccharide (0.5µg/ml) for 4 h, then together with Brefeldin A (300ng/ml) for another 20h. The nuclear counterstain is DAPI (blue). Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233551).
  • Flow Cytometry - Anti-IL-6 antibody [EPR22565-204] - BSA and Azide free (ab256355)
    Flow Cytometry - Anti-IL-6 antibody [EPR22565-204] - BSA and Azide free (ab256355)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HUVEC (Human umbilical vein endothelial cell) treated with 0.5ug/ml LPS for 4h, then together with 300ng/ml BFA for another 20h (Red) / Untreated control (Green), labeling IL-6 with ab233551 at 1/400 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233551).
  • Immunoprecipitation - Anti-IL-6 antibody [EPR22565-204] - BSA and Azide free (ab256355)
    Immunoprecipitation - Anti-IL-6 antibody [EPR22565-204] - BSA and Azide free (ab256355)

    IL-6 was immunoprecipitated from 0.35 mg HUVEC (Human umbilical vein endothelial cell) treated with 0.5µg/ml LPS for 4h, then together with 300ng/ml BFA for another 20h whole cell lysate with ab233551 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab233551 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

    Lane 1: HUVEC treated as above whole cell lysate 10 µg (Input).

    Lane 2: ab233551 IP in HUVEC treated as above whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233551 in HUVEC treated as above whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 15 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233551).

  • Anti-IL-6 antibody [EPR22565-204] - BSA and Azide free (ab256355)
    Anti-IL-6 antibody [EPR22565-204] - BSA and Azide free (ab256355)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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