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Human IL-13R alpha 2 ELISA Kit (ab193704)

Key features and details

  • Sensitivity: 0.4 ng/ml
  • Range: 0.4 ng/ml - 100 ng/ml
  • Sample type: Cell culture media, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich
  • Reacts with: Human

Overview

  • Product name

    Human IL-13R alpha 2 ELISA Kit
    See all IL-13 receptor alpha 2 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Serum, Plasma, Cell culture media

  • Assay type

    Sandwich

  • Sensitivity

    0.4 ng/ml

  • Range

    0.4 ng/ml - 100 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 77.79 71% - 85%
    Plasma 74.7 67% - 81%
    Cell culture media 81.45 72% - 89%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam's IL-13 Receptor alpha 2 Human ELISA Kit (ab193704) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-13 Receptor alpha 2 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for human IL-13 Receptor alpha 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IL-13 Receptor alpha 2 present in the samples. The wells are washed and biotinylated anti-human IL-13 Receptor alpha 2 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IL-13 Receptor alpha 2 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    Detection Antibody IL-13 Receptor alpha 2 (Biotinylated anti-Human IL-13 Receptor alpha 2) 2 vials
    Human IL-13R alpha 2 Standards (lyophilized) 2 vials
    Pre-coated Human IL-13R alpha 2 Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • Research areas

  • Function

    Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4).

  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 5 subfamily.
    Contains 1 fibronectin type-III domain.

  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • Cancer/testis antigen 19
    • CD213a2
    • CT19
    • I13R2_HUMAN
    • IL-13 receptor subunit alpha-2
    • IL-13R subunit alpha-2
    • IL-13R-alpha-2
    • IL-13RA2
    • IL13 receptor
    • IL13BP
    • IL13R
    • Il13ra2
    • Interleukin 13 binding protein
    • Interleukin 13 receptor alpha 2 chain
    • Interleukin-13 receptor subunit alpha-2
    • Interleukin-13-binding protein
    see all

  • Database links

    Images

    • Example Standard Curve
      Example Standard Curve

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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