Human IL-13R alpha 2 ELISA Kit (ab193704)
Key features and details
- Sensitivity: 0.4 ng/ml
- Range: 0.4 ng/ml - 100 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich
- Reacts with: Human
Overview
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Product name
Human IL-13R alpha 2 ELISA Kit
See all IL-13 receptor alpha 2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich -
Sensitivity
0.4 ng/ml -
Range
0.4 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 77.79 71% - 85% Plasma 74.7 67% - 81% Cell culture media 81.45 72% - 89% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam's IL-13 Receptor alpha 2 Human ELISA Kit (ab193704) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-13 Receptor alpha 2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for human IL-13 Receptor alpha 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IL-13 Receptor alpha 2 present in the samples. The wells are washed and biotinylated anti-human IL-13 Receptor alpha 2 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IL-13 Receptor alpha 2 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Detection Antibody IL-13 Receptor alpha 2 (Biotinylated anti-Human IL-13 Receptor alpha 2) 2 vials Human IL-13R alpha 2 Standards (lyophilized) 2 vials Pre-coated Human IL-13R alpha 2 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4). -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 5 subfamily.
Contains 1 fibronectin type-III domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Cancer/testis antigen 19
- CD213a2
- CT19
see all -
Database links
- Entrez Gene: 3598 Human
- Omim: 300130 Human
- SwissProt: Q14627 Human
- Unigene: 336046 Human
Images
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Example Standard Curve
