Anti-IL-32 antibody (ab37158)
Key features and details
- Rabbit polyclonal to IL-32
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-IL-32 antibody
See all IL-32 primary antibodies -
Description
Rabbit polyclonal to IL-32 -
Host species
Rabbit -
Specificity
ab37158 recognises all 4 isoforms of IL-32 (100% sequence alignment to each). -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human IL-32 (C terminal).
(Peptide available asab39778)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-IL-32 antibody (ab37158)Lane 1 : Anti-IL-32 antibody (ab37158) at 2 µg/ml
Lane 2 : Anti-IL-32 antibody (ab37158) at 4 µg/ml
All lanes : Human spleen tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 26 kDa
-
Western blot - Anti-IL-32 antibody (ab37158)Lane 1 : Anti-IL-32 antibody (ab37158) at 5 µg/ml
Lane 2 : Anti-IL-32 antibody (ab37158) at 10 µg/ml
All lanes : Human spleen cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 26 kDa
Observed band size: 12,26 kDa why is the actual band size different from the predicted?
Two isoforms of IL32 can be seen -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-32 antibody (ab37158)Immunohistochemical analysis of IL32 expression in mouse spleen tissue with ab37158 at 2µg/ml -
Western blot - Anti-IL-32 antibody (ab37158)Westen blot data for human spleen cell lysates (left lanes) and mouse EL4 cell lysates (right lanes). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-32 antibody (ab37158)IHC-P of Human spleen tissue with ab37158 at 10 µg/ml -
Immunocytochemistry/ Immunofluorescence - Anti-IL-32 antibody (ab37158)Immunofluorescence of IL-32 in Human Spleen cells using ab37158 at 20 ug/ml.
