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Mouse IL-31 ELISA Kit (ab243681)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 24 pg/ml
  • Range: 78.1 pg/ml - 5000 pg/ml
  • Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse IL-31 ELISA Kit
    See all IL-31 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    serum 8 5.1%
    Inter-assay
    Sample n Mean SD CV%
    serum 3 6%

  • Sample type

    Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    24 pg/ml

  • Range

    78.1 pg/ml - 5000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 90 84% - 97%
    Cell culture media 90 83% - 94%
    Hep Plasma 91 87% - 98%
    EDTA Plasma 99 95% - 104%
    Cit plasma 84 80% - 91%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse IL-31 ELISA Kit (ab243681) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-31 protein in cit plasma, edta plasma, hep plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-31 with 24 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse IL-31 ELISA Kit (ab243681)
    Other - Mouse IL-31 ELISA Kit (ab243681)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of mouse IL-31 standard curve in Sample Diluent 50BP.
    Example of mouse IL-31 standard curve in Sample Diluent 50BP.

    The IL-31 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

     

     

  • Interpolated concentrations of spike IL-31 in mouse serum, plasma and cell culture supernatant samples.
    Interpolated concentrations of spike IL-31 in mouse serum, plasma and cell culture supernatant samples.

    The concentrations of IL-31 were measured in duplicates, interpolated from the IL-31 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (EDTA) 12.5%, plasma (citrate) 12.5%, plasma (heparin) 12.5% and RPMI media 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

     

  • Sandwich ELISA - Mouse IL-31 ELISA Kit (ab243681)
    Sandwich ELISA - Mouse IL-31 ELISA Kit (ab243681)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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