Anti-IL-23 antibody (ab45420)
Key features and details
- Rabbit polyclonal to IL-23
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-23 antibody
See all IL-23 primary antibodies -
Description
Rabbit polyclonal to IL-23 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IL-23 (C terminal). 14 amino acid peptide located within the last 50 amino acids of IL23.
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Positive control
- Raji cells and Raji cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-23 antibody (ab45420)
Immunohistochemistry of IL-23 in human pancreas tissue with ab45420 at 5 µg/mL.
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Western blot - Anti-IL-23 antibody (ab45420)All lanes : Anti-IL-23 antibody (ab45420) at 1 µg/ml
Lane 1 : Raji lysate
Lane 2 : Raji lysate with IL23 P19 blocking peptide
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-IL23 antibody (ab45420) This image is courtesy of an anonymous AbreviewAb45420 staining IL23 in Mouse bone marrow derived macrophages by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X100 and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody at 1/200 dilution in 5% goat serum in PBS for 1 hour at 20°C. An Alexa Fluor® 488 goat anti-rabbit was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-IL-23 antibody (ab45420)ab45420 at 10 µg/ml staining IL23 in Raji cells by ICC/IF -
Immunocytochemistry/ Immunofluorescence - Anti-IL-23 antibody (ab45420) This image is courtesy of an Abreview submitted by Dr James Harrisab45420 staining IL23 P19 (green) in Human THP-1 cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in 5% goat serum + 1 BSA) for 1 hour at 20°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-IL-23 antibody (ab45420)Immunofluorescence of IL-23 in Raji cells using ab45420 at 20 ug/ml.
