ab84472 used in Neutralisation.A = untreatedB = treated with IL-2 (10ng/ml) + IL-23 (10ng)C = treated with IL-2 (10ng/ml) + IL-23 (50ng/ml)D = treated with anti-IL-23 (5µg/ml) + IL-2 + IL-23 (50ng/ml)Human monocytes were fixed in methanol, permeabilized using 0.1% Saponin/ PBS, blocked with 4% serum for 30 minutes at 25°C and then incubated with ab84471 at 5µg/ml for 48 hours at 37°C.Staining: IL-17 (red) GFP (green).We have examined the effect of IL-23 and in combination with anti-IL-23 on IL-17 production in monocytes. IL-23 leads to increased IL-17 secretion (Figure B and C) and this effect was abolished with anti-IL-23 (Figure D). IL-2 is part of our culture medium for monocytes. IL-2 stimulation serves as a basis to keep the cell alive. Thus almost every cytokine that we used is used in combination with IL-2. Because it is part of the medium, IL-2 can be omitted when specifying the stimulation. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab84471).