Anti-EBI3 antibody [EPR23372-282] - BSA and Azide free (ab272937)
![Anti-EBI3 antibody [EPR23372-282] - BSA and Azide free (ab272937)](https://www.abcam.com/ps/products/272/ab272937/Images/ab272937-1-anti-ebi3-antibody-epr23372282-immunohistochemistry-human-placenta.jpg "Anti-EBI3 antibody [EPR23372-282] - BSA and Azide free (ab272937)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23372-282] to EBI3 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-EBI3 antibody [EPR23372-282] - BSA and Azide free
See all EBI3 primary antibodies -
Description
Rabbit monoclonal [EPR23372-282] to EBI3 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human placenta lysate. His-tagged recombinant human EBI3 protein. IHC-P: Human placenta, tonsil and Human colon carcinoma tissues.
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General notes
ab272937 is the carrier-free version of ab272709. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23372-282 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [EPR23372-282] - BSA and Azide free (ab272937)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling EBI3 with ab272709 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human placenta (PMID: 21849417). The section was incubated with ab272709 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272709).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [EPR23372-282] - BSA and Azide free (ab272937)](https://www.abcam.com/ps/products/272/ab272937/Images/ab272937-2-anti-ebi3-antibody-epr23372282-immunohistochemistry-human-colon-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [EPR23372-282] - BSA and Azide free (ab272937)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling EBI3 with ab272709 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human colon carcinoma (PMID: 24040445). The section was incubated with ab272709 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272709).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [EPR23372-282] - BSA and Azide free (ab272937)](https://www.abcam.com/ps/products/272/ab272937/Images/ab272937-3-anti-ebi3-antibody-epr23372282-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [EPR23372-282] - BSA and Azide free (ab272937)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling EBI3 with ab272709 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on scattered cells in human tonsil (PMID: 16639698). The section was incubated with ab272709 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272709).
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![Anti-EBI3 antibody [EPR23372-282] - BSA and Azide free (ab272937)](https://www.abcam.com/ps/products/272/ab272937/Images/ab272937-4-benefits-of-recombinant-antibodies.png)
Anti-EBI3 antibody [EPR23372-282] - BSA and Azide free (ab272937)
