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Anti-IKK alpha antibody [ABM10G9] (ab227852)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-IKK alpha antibody [ABM10G9] (ab227852)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [ABM10G9] to IKK alpha
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-IKK alpha antibody [ABM10G9]
    See all IKK alpha primary antibodies
  • Description

    Mouse monoclonal [ABM10G9] to IKK alpha
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Positive control

    • WB: HeLa, MCF7, U-87 MG, PC-3 and HEK-293 cell lysates; mouse small intestine lysate. Flow Cyt: Jurkat, HEK-293 and HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    ABM10G9
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • NFkB
    • IKK
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • NFkB
    • IKK

Images

  • Western blot - Anti-IKK alpha antibody [ABM10G9] (ab227852)
    Western blot - Anti-IKK alpha antibody [ABM10G9] (ab227852)
    All lanes : Anti-IKK alpha antibody [ABM10G9] (ab227852) at 2 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate
    Lane 4 : Mouse small intestine lysate
    Lane 5 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
    Lane 6 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

    Predicted band size: 85 kDa

  • Flow Cytometry - Anti-IKK alpha antibody [ABM10G9] (ab227852)
    Flow Cytometry - Anti-IKK alpha antibody [ABM10G9] (ab227852)

    Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling IKK alpha with ab227852 at 0.5 µg/106 cells (red) compared with an isotype control (green). Goat anti-mouse PE conjugate was used as secondary antibody.

  • Flow Cytometry - Anti-IKK alpha antibody [ABM10G9] (ab227852)
    Flow Cytometry - Anti-IKK alpha antibody [ABM10G9] (ab227852)

    Flow cytometric analysis of HEK-293 (human epithelial cell line from embryonic kidney) cell line labeling IKK alpha with ab227852 at 0.5 µg/106 cells (red) compared with an isotype control (green). Goat anti-mouse PE conjugate was used as secondary antibody.

  • Flow Cytometry - Anti-IKK alpha antibody [ABM10G9] (ab227852)
    Flow Cytometry - Anti-IKK alpha antibody [ABM10G9] (ab227852)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling IKK alpha with ab227852 at 0.5 µg/106 cells (red) compared with an isotype control (green). Goat anti-mouse PE conjugate was used as secondary antibody.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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