Anti-BID (phospho S78) antibody (ab264131)
Key features and details
- Rabbit polyclonal to BID (phospho S78)
- Suitable for: WB, IP
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-BID (phospho S78) antibody -
Description
Rabbit polyclonal to BID (phospho S78) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse
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Immunogen
Synthetic peptide within Mouse BID (phospho S78). The exact sequence is proprietary.
Database link: P70444 -
Positive control
- WB: NIH/3T3 whole cell lysate treated with Etoposide. IP: NIH/3T3 whole cell lysate treated with Etoposide.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-BID (phospho S78) antibody (ab264131)All lanes : Anti-BID (phospho S78) antibody (ab264131) at 1 µg/ml
Lane 1 : NIH/3T3 whole cell lysate untreated at 50 µg
Lane 2 : NIH/3T3 whole cell lysate treated with Etoposide (100 µM , 3 hrs) at 50 µg
Lane 3 : NIH/3T3 whole cell lysate untreated at 80 µg
Lane 4 : NIH/3T3 whole cell lysate treated with Etoposide (100 µM , 3 hrs) at 80 µg
Predicted band size: 22 kDa
Exposure time: 3 minutes4-20% SDS-PAGE.
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Immunoprecipitation - Anti-BID (phospho S78) antibody (ab264131)Whole cell lysate (1 mg for IP, 20% of IP loaded) from NIH/3T3 cells treated with 100 μM etoposide for 3 hours (+) or mock treated (-) cells. ab264131 was used for WB at 1 μg/ml. An alternative Rabbit anti-BID antibody was used for IP for BID and phospho-BID.