Anti-COLEC11 antibody [821] (ab278063)
![Anti-COLEC11 antibody [821] (ab278063)](https://www.abcam.com/ps/products/278/ab278063/Images/ab278063-445340-anti-collectin-11-antibodu-821-icc-cho-cells.png "Anti-COLEC11 antibody [821] (ab278063)")
Key features and details
- Mouse monoclonal [821] to COLEC11
- Suitable for: WB, ICC, ELISA
- Reacts with: Chinese hamster
- Isotype: IgG1
Overview
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Product name
Anti-COLEC11 antibody [821]
See all COLEC11 primary antibodies -
Description
Mouse monoclonal [821] to COLEC11 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC, ELISAmore details -
Species reactivity
Reacts with: Chinese hamster -
Immunogen
Recombinant fragment corresponding to Human COLEC11 aa 107-271. Corresponding to the carbohydrate recognition domain (CRD).
Database link: Q9BWP8 -
Positive control
- WB: CHO cells. ICC: CHO cells. ELISA: CHO cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 5.00
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
821 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry - Anti-COLEC11 antibody [821] - BSA and Azide free (ab278063)
Immunocytochemical analysis of CHO cells labeling collectin-11 with ab278063 at 1/500 dilution (Green). The FITC-conjugate rabbit anti-mouse IgG at 1/4000 dilution was added. Nucleus (DNA) was stained with DAPI (Blue). On panel A CHO cells were expressing human recombinant Collectin-11. On panel B CHO cells were mock infected.
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Western blot - Anti-COLEC11 antibody (ab278063)Anti-COLEC11 antibody [821] (ab278063) at 1/500 dilution + Supernatant of CHO cells expressing human recombinant
Collectin-11 at 1 µg/ml
Predicted band size: 29 kDa
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ELISA - Anti-COLEC11 antibody (ab278063)The indicated amounts of recombinant Collectin-11 protein expressed in CHO cells were coated onto the wells of the ELISA plate. After blocking with 5% skim milk, ab278063 at 1/1000 dilution was added to each well. HRP-conjugated goat anti-mouse IgG 100 µl, at 1/4000 dilution was added. As substrate, orthophenylenediamine was used. Optical density (OD) measured at 490nm.
