Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LSM12 antibody [EPR12282-87] (ab173292)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12282-87] to LSM12
  • Suitable for: ICC/IF, IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-LSM12 antibody [EPR12282-87]
    See all LSM12 primary antibodies

  • Description

    Rabbit monoclonal [EPR12282-87] to LSM12

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human LSM12. The exact sequence is proprietary.
    Database link: Q3MHD2

  • Positive control

    • 293T, HepG2, HeLa and fetal brain lysates; Human endometrial carcinoma tissue; HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12282-87

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-LSM12 antibody [EPR12282-87] (ab173292)
    Western blot - Anti-LSM12 antibody [EPR12282-87] (ab173292)
    All lanes : Anti-LSM12 antibody [EPR12282-87] (ab173292) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM12 antibody [EPR12282-87] (ab173292)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM12 antibody [EPR12282-87] (ab173292)

    Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling LSM12 with ab173292 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-LSM12 antibody [EPR12282-87] (ab173292)
    Immunocytochemistry/ Immunofluorescence - Anti-LSM12 antibody [EPR12282-87] (ab173292)

    Immunofluorescent analysis of HepG2 cells labeling LSM12 with ab173292 at 1/100 dilution.

  • Immunoprecipitation - Anti-LSM12 antibody [EPR12282-87] (ab173292)
    Immunoprecipitation - Anti-LSM12 antibody [EPR12282-87] (ab173292)

    Western blot analysis on immunoprecipitation pellet from 1) HeLa cell lysate or 2) 1X PBS using ab173292 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-LSM12 antibody [EPR12282-87] (ab173292)
    Anti-LSM12 antibody [EPR12282-87] (ab173292)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-LSM12 antibody [EPR12282-87] (ab173292)

© 2021 Astana Biomed Group LLP. All rights reserved.