Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Interferon alpha 2 antibody [EPR19074] - BSA and Azide free (ab230830)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19074] to Interferon alpha 2 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IP, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-Interferon alpha 2 antibody [EPR19074] - BSA and Azide free
    See all Interferon alpha 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR19074] to Interferon alpha 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IP, ICCmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HEK-293T cells transfected with Interferon alpha 2 expression vector containing a GFP-Myc-tag / GFP-tag.

  • General notes

    Ab230830 is the carrier-free version of ab196221. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab230830 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Interferon alpha 2 antibody [EPR19074] - BSA and Azide free (ab230830)
    Immunoprecipitation - Anti-Interferon alpha 2 antibody [EPR19074] - BSA and Azide free (ab230830)

    Interferon alpha 2 was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (transfected with Interferon alpha 2 expression vector containing a GFP-His-tag) whole cell lysate using ab196221 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab196221 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as for detection at 1/10000 dilution.

    Lane 1: HEK-293T (transfected with Interferon alpha 2 expression vector containing a GFP-His-tag) whole cell lysate 10 μg (Input).

    Lane 2: ab196221 IP in HEK-293T (transfected with Interferon alpha 2 expression vector containing a GFP-His-tag) whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab196221 inHEK-293T (transfected with Interferon alpha 2 expression vector containing a GFP-His-tag) whole cell lysate 

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

    The molecular mass observed is due to the presence of the GFP epitope tag on full length IFN alpha 2.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196221).

  • Flow Cytometry - Anti-Interferon alpha 2 antibody [EPR19074] - BSA and Azide free (ab230830)
    Flow Cytometry - Anti-Interferon alpha 2 antibody [EPR19074] - BSA and Azide free (ab230830)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (transfected with Interferon alpha 2 expression vector containing a GFP-His-tag cell line) cells labeling Interferon alpha 2 with ab196221 at 1/600 (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)(black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196221).

  • Immunocytochemistry - Anti-Interferon alpha 2 antibody [EPR19074] - BSA and Azide free (ab230830)
    Immunocytochemistry - Anti-Interferon alpha 2 antibody [EPR19074] - BSA and Azide free (ab230830)

    Immunocytochemical analysis of agarose-embedded HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (transfected with Interferon alpha 2 (pcDNA3.1 (+)-EGFP-Myc)) cells labeling Interferon alpha 2 with ab196221 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use secondary antibody. Positive staining on HEK-293T cells transfected with Interferon alpha 2 (pcDNA3.1 (+)-EGFP-Myc) (image A); and no staining on HEK-293T cells without transfection (image B). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of ab196221, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196221).

  • Immunocytochemistry/ Immunofluorescence - Anti-Interferon alpha 2 antibody [EPR19074] - BSA and Azide free (ab230830)
    Immunocytochemistry/ Immunofluorescence - Anti-Interferon alpha 2 antibody [EPR19074] - BSA and Azide free (ab230830)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (transfected with either GFP-tagged Interferon alpha 2 expression vector or empty GFP expression vector) cells labeling Interferon alpha 2 with ab196221 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic staining in HEK-293T cells transfected with GFP-tagged Interferon alpha 2 expression vector. 

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of ab196221, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196221).

     

  • Anti-Interferon alpha 2 antibody [EPR19074] - BSA and Azide free (ab230830)
    Anti-Interferon alpha 2 antibody [EPR19074] - BSA and Azide free (ab230830)

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