Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGFR antibody [EGFR1] - BSA and Azide free (ab264523)

Key features and details

  • Mouse monoclonal [EGFR1] to EGFR - BSA and Azide free
  • Suitable for: IHC-Fr, ICC/IF, IP, Flow Cyt, IHC - Wholemount
  • Reacts with: Horse, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-EGFR antibody [EGFR1] - BSA and Azide free
    See all EGFR primary antibodies

  • Description

    Mouse monoclonal [EGFR1] to EGFR - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, IP, Flow Cyt, IHC - Wholemountmore details
    Unsuitable for: ELISA or WB

  • Species reactivity

    Reacts with: Horse, Human

  • Immunogen

    Tissue, cells or virus corresponding to Human EGFR (extracellular). Human epidermoid carcinoma line A431; epitope mapped between aa 6-273 of human EGFR.

  • Epitope

    Extracellular.

  • Positive control

    • IHC-Fr: Frozen normal human placenta ICC/IF: A431 cells Flow Cyt: A 431 cells

  • General notes

    Ab264523 is a PBS only version of ab30.

    Recognises the external EGF-binding domain of the EGFR transmembrane glycoprotein. No effect on tyrosine kinase activity of EGFR.

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-EGFR antibody [EGFR1] - BSA and Azide free (ab264523)
    Flow Cytometry - Anti-EGFR antibody [EGFR1] - BSA and Azide free (ab264523)

    Overlay histograms showing left positive A431 cells and right negative Jurkat cells stained with ab30 (red line). The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab30, 1x106 cells in 100ul at 1ug/ml) for 30 min on ice. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) preabsorbed (ab150117) at 1/2000 dilution for 30 min on ice.

    Isotype control antibody (black line) was mouse IgG2bκ (ab170192) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab30).

  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EGFR1] - BSA and Azide free (ab264523)
    Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EGFR1] - BSA and Azide free (ab264523)

    ab30 staining EGFR (colored green) in A431 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab30 at 5μg/ml overnight at 4ºC. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab30).

  • Immunohistochemistry (Frozen sections) - Anti-EGFR antibody [EGFR1] - BSA and Azide free (ab264523)
    Immunohistochemistry (Frozen sections) - Anti-EGFR antibody [EGFR1] - BSA and Azide free (ab264523)

    IHC image of EGFR staining in a section of frozen normal human placenta, fixed in 10% paraformaldehyde (10 min). Staining was performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab30, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab30).

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