Anti-DAB2 antibody (ab137866)
Key features and details
- Rabbit polyclonal to DAB2
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-DAB2 antibody
See all DAB2 primary antibodies -
Description
Rabbit polyclonal to DAB2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse DAB2 aa 300-400 conjugated to keyhole limpet haemocyanin.
Database link: P98078 -
Positive control
- This antibody gave a positive signal in Raw264.7 whole cell lysate as well as the following Mouse tissue lysates: E10 Whole Embryo; E10 Embryonic Brain.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatHorseCowHumanPigChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 96 kDa (predicted molecular weight: 82 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 96 kDa (predicted molecular weight: 82 kDa).Target
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Relevance
DAB2 is a component of the CSF1 signal transduction pathway. DAB2 mRNA is expressed in normal ovarian epithelial cells but is down regulated or absent from ovarian carcinoma cell lines. The down regulation of DAB2 may play an important role in ovarian carcinogenesis. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2 like domains, alpha and beta). -
Cellular localization
Cytoplasmic vesicle; clathrin coated vesicle; clathrin coated vesicle membrane. Cell membrane; coated pit; clathrin coated pit. Note: Colocalizes with large insert containing isoforms of MYO6 at clathrin coated pits/vesicles. -
Database links
- Entrez Gene: 1601 Human
- Entrez Gene: 13132 Mouse
- Entrez Gene: 79128 Rat
- Omim: 601236 Human
- SwissProt: P98082 Human
- SwissProt: P98078 Mouse
- SwissProt: O88797 Rat
- Unigene: 696631 Human
see all -
Alternative names
- DAB 2 antibody
- Dab, mitogen-responsive phosphoprotein, homolog 2 (Drosophila) antibody
- DAB2 antibody
see all
Images
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Western blot - Anti-DAB2 antibody (ab137866)Anti-DAB2 antibody (ab137866) at 1 µg/ml (Milk blocking 3%) + RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 82 kDa
Observed band size: 96 kDa why is the actual band size different from the predicted?
Additional bands at: 68 kDa (possible non-specific binding)
Exposure time: 4 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab137866 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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Western blot - Anti-DAB2 antibody (ab137866)All lanes : Anti-DAB2 antibody (ab137866) at 1 µg/ml (Milk blocking 3%)
Lane 1 : Mouse Embryo E10 Tissue Lysate
Lane 2 : E10 Mouse Embryo Brain Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 82 kDa
Observed band size: 96 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa (possible non-specific binding), 35 kDa (possible non-specific binding)
Exposure time: 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab137866 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (1)
ab137866 has been referenced in 1 publication.
- Lu M et al. miR-149 promotes the myocardial differentiation of mouse bone marrow stem cells by targeting Dab2. Mol Med Rep 17:8502-8509 (2018). PubMed: 29693140
Images
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Western blot - Anti-DAB2 antibody (ab137866)Anti-DAB2 antibody (ab137866) at 1 µg/ml (Milk blocking 3%) + RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 82 kDa
Observed band size: 96 kDa why is the actual band size different from the predicted?
Additional bands at: 68 kDa (possible non-specific binding)
Exposure time: 4 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab137866 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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Western blot - Anti-DAB2 antibody (ab137866)All lanes : Anti-DAB2 antibody (ab137866) at 1 µg/ml (Milk blocking 3%)
Lane 1 : Mouse Embryo E10 Tissue Lysate
Lane 2 : E10 Mouse Embryo Brain Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 82 kDa
Observed band size: 96 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa (possible non-specific binding), 35 kDa (possible non-specific binding)
Exposure time: 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab137866 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
