Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free (ab256545)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22881-69] to DAB2 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free
    See all DAB2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22881-69] to DAB2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: PC-3, HeLa and Caco-2 lysates. IHC-P: Human placenta, Human bladder cancer, Mouse kidney and Rat kidney tissues. IP: HeLa cell lysate

  • General notes

    Ab256545 is the carrier-free version of ab256524. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256545 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free (ab256545)
    Western blot - Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free (ab256545)
    All lanes : Anti-DAB2 antibody [EPR22881-69] (ab256524) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : DAB2 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Observed band size: 95 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab256524, the same antibody clone in a different buffer formulation.

    Lanes 1-3: Merged signal (red and green). Green - ab256524 observed at 95 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab256524 Anti-DAB2 antibody [EPR22881-69]  was shown to specifically react with DAB2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265731 (knockout cell lysate ab257910) was used. Wild-type and DAB2 knockout samples were subjected to SDS-PAGE. ab256524 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free (ab256545)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free (ab256545)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling DAB2 with ab256524 at 1/500 dilution (0.14 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat kidney (PMID: 30052485) is observed. The section was incubated with ab256524 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256524).

  • Immunoprecipitation - Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free (ab256545)
    Immunoprecipitation - Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free (ab256545)

    DAB2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab256524 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256524 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg

    Lane 2: ab256524 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256490 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256524).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free (ab256545)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free (ab256545)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling DAB2 with ab256524 at 1/500 dilution (0.14ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse kidney (PMID: 30052485) is observed. The section was incubated with ab256524 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256524).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free (ab256545)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free (ab256545)

    Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling DAB2 with ab256524 at 1/500 dilution (0.14ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human bladder cancer (PMID:17671122) is observed. The section was incubated with ab256524 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256524).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free (ab256545)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free (ab256545)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling DAB2 with ab256524 at 1/500 dilution (0.14ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human placenta (PMID:27072056) is observed. The section was incubated with ab256524 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256524).

  • Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free (ab256545)
    Anti-DAB2 antibody [EPR22881-69] - BSA and Azide free (ab256545)

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