Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11767(B)] to IFI16
  • Suitable for: ICC/IF, IHC-P, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-IFI16 antibody [EPR11767(B)]
    See all IFI16 primary antibodies

  • Description

    Rabbit monoclonal [EPR11767(B)] to IFI16

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in Human IFI16 (UniProt ID: Q16666).

  • Positive control

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

    ab169788 showing -ve staining in Human gastric adenocarcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
    Western blot - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
    All lanes : Anti-IFI16 antibody [EPR11767(B)] (ab169788) at 1/1000 dilution

    Lane 1 : U937 treated with IFN gamma cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 88 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
    Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
    Immunofluorescence analysis of Raji cells labeling IFI16, using ab169788 at a 1/100 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
    Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) cells labelling IFI16 with ab169788 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

    The image shows nuclear staining on Jurkat cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling IFI16, using ab169788 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bone marrow tissue labeling IFI16, using ab169788 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

    ab169788 showing +ve staining in Human normal spleen tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

    ab169788 showing -ve staining in Human normal uterus tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
    Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

    Flow cytometry analysis of Raji (human Burkitt's lymphoma) cells labelling IFI16 (red) with ab169788 at dilution of 1/500. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde. Isotype control antibody was (ab172730) Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
    Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

    Flow cytometry analysis of Jurkat (human acute T cell leukemia) cells labelling IFI16 (red) with ab169788 at dilution of 1/500. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde. Isotype control antibody was (ab172730) Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • Anti-IFI16 antibody [EPR11767(B)] (ab169788)
    Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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