Anti-CALML3 antibody (ab275579)
Key features and details
- Rabbit polyclonal to CALML3
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CALML3 antibody
See all CALML3 primary antibodies -
Description
Rabbit polyclonal to CALML3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein (His-tag) corresponding to Human CALML3 aa 1 to the C-terminus.
Database link: P27482 -
Positive control
- IHC-P: Human breast carcinoma tissue. WB: A431 whole cell lysate. IP: A431 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CALML3 antibody (ab275579)Anti-CALML3 antibody (ab275579) at 1/500 dilution + A431 (Human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Performed under reducing conditions.Developed using the Odyssey technique.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CALML3 antibody (ab275579)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue (from 4 donors) labeling CALML3 using ab275579 at a 1/1000 dilution.
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Immunoprecipitation - Anti-CALML3 antibody (ab275579)CALML3 was immunoprecipitated using 0.5 mg A431 (Human epidermoid carcinoma cell line) whole cell lysate, ab275579 and 15µl of 50 % Protein G agarose.
ab275579 used at 1/1000 dilution, followed by a Dylight 800-labeled secondary antibody to rabbit IgG (H+L), at 1/5000 dilution.
Developed using the odssey technique.
Predicted band size: 17 kDa
Observed band size: 17 kDa