Human LIF ELISA Kit (ab242228)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.469 pg/ml
- Range: 23.44 pg/ml - 1500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human LIF ELISA Kit
See all LIF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% cell culture 8 6.1% Inter-assay Sample n Mean SD CV% cell culture 3 10.3% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.469 pg/ml -
Range
23.44 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 102 99% - 111% Serum 89 85% - 93% Hep Plasma 94 87% - 92% EDTA Plasma 89 87% - 92% Cit plasma 89 89% - 91% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human LIF ELISA Kit (ab242228) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LIF protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LIF with 1.469 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human LIF Capture Antibody 1 x 600µl 10X Human LIF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human LIF Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. -
Sequence similarities
Belongs to the LIF/OSM family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CDF
- Cholinergic Differentiation Factor
- D factor
see all -
Database links
- Entrez Gene: 3976 Human
- Omim: 159540 Human
- SwissProt: P15018 Human
- Unigene: 2250 Human
Images
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Other - Human LIF ELISA Kit (ab242228)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human LIF Standard Curve Comparison.Standard Curve comparison between human LIF SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human LIF standard curve in Sample Diluent NS.The LIF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native LIF in human cell culture supernatant samples.The concentrations of LIF were measured in duplicates, interpolated from the LIF standard curves and corrected for sample dilution. Undiluted samples are 1.5% PHA-M treated PBMC cell culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIF concentration was determined to be 8336.44 pg/mL in 1.5% PHA-M treated PBMC cell culture supernatant. -
Sandwich ELISA - Human LIF ELISA Kit (ab242228)To learn more about the advantages of recombinant antibodies see here.
