Anti-ID3 antibody (ab86017)
Key features and details
- Rabbit polyclonal to ID3
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-ID3 antibody
See all ID3 primary antibodies -
Description
Rabbit polyclonal to ID3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
3 synthetic peptides (Human) conjugated to KLH
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Positive control
- Recombinant protein produced in an induced culture of E.coli.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ID3 antibody (ab86017)All lanes : Anti-ID3 antibody (ab86017) at 1/1000 dilution
Lane 1 : Uninduced (negative control) culture of E.coli
Lane 2 : Induced culture of E.coli
Secondary
All lanes : Anti-Rabbit IgG HRP conjugated at 1/1000 dilution
Predicted band size: 13 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Additional bands at: 124 kDa, 209 kDa, 32 kDa, 45 kDa, 75 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 10%Predicted molecular weight of recombinant ID3:27kDa (13kDa + another 14kDa for the tag).
