Antibodies, Proteins, Kits and Reagents for Life Science

241 228 ₸ Product size

100 µl 241 228 ₸

Order now and get it on Tuesday March 09, 2021

Anti-TMP21 antibody [EPR9037(B)] (ab133771)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9037(B)] to TMP21
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-TMP21 antibody [EPR9037(B)]
See all TMP21 primary antibodies
Description

Rabbit monoclonal [EPR9037(B)] to TMP21
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human TMP21 aa 100-200. The exact sequence is proprietary.
Positive control
- U-87 MG, Raji, HeLa, 293T, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 2.39 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9037(B)
Isotype

IgG
Research areas

Western blot - Anti-TMP21 antibody [EPR9037(B)] (ab133771)

All lanes : Anti-TMP21 antibody [EPR9037(B)] (ab133771) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : TMED10 knockout HEK293T cell lysate
Lane 3 : U-87 MG cell lysate
Lane 4 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 25 kDa
Observed band size: 19 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab133771 observed at 19 kDa. Red - loading control ab8245 observed at 36 kDa.

ab133771 Anti-TMP21 antibody [EPR9037(B)] was shown to specifically react with TMP21 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266227 (knockout cell lysate ab258233) was used. Wild-type and TMP21 knockout samples were subjected to SDS-PAGE. ab133771 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-TMP21 antibody [EPR9037(B)] (ab133771)

All lanes : Anti-TMP21 antibody [EPR9037(B)] (ab133771) at 1/1000 dilution

Lane 1 : U-87 MG cell lysate
Lane 2 : Raji cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T cell lysate
Lane 5 : C6 cell lysate
Lane 6 : RAW 264.7 cell lysate
Lane 7 : PC12 cell lysate
Lane 8 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 25 kDa
OI-RD Scanning - Anti-TMP21 antibody [EPR9037(B)] (ab133771)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-TMP21 antibody [EPR9037(B)] (ab133771)

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