Feline IL-8 ELISA Kit (ab193688)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 25 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cat
Overview
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Product name
Feline IL-8 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
25 pg/ml -
Range
25 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 89.71 82% - 97% Plasma 84.6 71% - 95% Cell culture media 118 100% - 149% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cat -
Product overview
Abcam’s IL-8 Feline ELISA Kit (ab193688) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of feline IL-8 in serum, plasma (feline IL8 concentration is pretty low in normal plasma, it may not be detected in this assay) and cell culture supernatant.
This assay employs an antibody specific for feline IL-8 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IL-8 present in the samples. The wells are washed and biotinylated anti-feline IL8 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IL-8 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated feline IL-8 detection antibody (lyophilized) 2 vials feline IL-8 Standards (lyophilized) 2 vials Pre-coated feline IL-8 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
Cellular localization
Secreted -
Alternative names
- Alveolar macrophage chemotactic factor I
- Beta endothelial cell derived neutrophil activating peptide
- Beta thromboglobulin like protein
see all -
Database links
- Entrez Gene: 493836 Cat
- SwissProt: Q9XSX5 Cat
Images
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Standard Curve.
Standard Curve using ab193688 IL-8 Feline ELISA Kit.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.