Anti-ID3 antibody [OTI8B3] (ab236505)
![Anti-ID3 antibody [OTI8B3] (ab236505)](https://www.abcam.com/ps/products/236/ab236505/Images/ab236505-313068-anti-id3-antibody-oti8b3-western-blot.jpg "Anti-ID3 antibody [OTI8B3] (ab236505)")
Key features and details
- Mouse monoclonal [OTI8B3] to ID3
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-ID3 antibody [OTI8B3]
See all ID3 primary antibodies -
Description
Mouse monoclonal [OTI8B3] to ID3 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Recombinant fragmentIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human ID3 aa 1 to the C-terminus. (NP_002158) produced in HEK-293T cells.
Database link: Q02535 -
Positive control
- WB: HEK-293T transfected with pCMV6-ENTRY ID3 cDNA, whole cell lysate. IHC-P: Human colon, kidney, liver, ovary, thyroid and prostate tissue. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY ID3 cDNA. Flow: HeLa and Jurkat cells.
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General notes
Clone OTI8B3 (formerly 8B3).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI8B3 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-ID3 antibody [OTI8B3] (ab236505)All lanes : Anti-ID3 antibody [OTI8B3] (ab236505) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control cDNA, whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY ID3 cDNA, whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 13 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (ab236505)](https://www.abcam.com/ps/products/236/ab236505/Images/ab236505-313070-anti-id3-antibody-oti8b3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (ab236505)Paraffin-embedded human colon tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (ab236505)](https://www.abcam.com/ps/products/236/ab236505/Images/ab236505-313071-anti-id3-antibody-oti8b3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (ab236505)Paraffin-embedded human kidney tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (ab236505)](https://www.abcam.com/ps/products/236/ab236505/Images/ab236505-313073-anti-id3-antibody-oti8b3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (ab236505)Paraffin-embedded human liver tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (ab236505)](https://www.abcam.com/ps/products/236/ab236505/Images/ab236505-313074-anti-id3-antibody-oti8b3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (ab236505)Paraffin-embedded human ovary tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (ab236505)](https://www.abcam.com/ps/products/236/ab236505/Images/ab236505-313075-anti-id3-antibody-oti8b3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (ab236505)Paraffin-embedded human thyroid tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (ab236505)](https://www.abcam.com/ps/products/236/ab236505/Images/ab236505-313077-anti-id3-antibody-oti8b3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (ab236505)Paraffin-embedded human prostate tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.
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![Immunocytochemistry/ Immunofluorescence - Anti-ID3 antibody [OTI8B3] (ab236505)](https://www.abcam.com/ps/products/236/ab236505/Images/ab236505-313078-anti-id3-antibody-oti8b3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ID3 antibody [OTI8B3] (ab236505)COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY ID3 cDNA stained for ID3 (green) using ab236505 at 1/100 dilution in ICC/IF.
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![Flow Cytometry - Anti-ID3 antibody [OTI8B3] (ab236505)](https://www.abcam.com/ps/products/236/ab236505/Images/ab236505-313079-anti-id3-antibody-oti8b3-flow-cytometry.jpg)
Flow Cytometry - Anti-ID3 antibody [OTI8B3] (ab236505)Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling ID3 with ab236505 at 1/100 dilution (red) compared to a negative control antibody (blue).
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![Flow Cytometry - Anti-ID3 antibody [OTI8B3] (ab236505)](https://www.abcam.com/ps/products/236/ab236505/Images/ab236505-313080-anti-id3-antibody-oti8b3-flow-cytometry.jpg)
Flow Cytometry - Anti-ID3 antibody [OTI8B3] (ab236505)Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling ID3 with ab236505 at 1/100 dilution (red) compared to a negative control antibody (blue).
