Anti-Iba1 antibody (ab48004)
Key features and details
- Goat polyclonal to Iba1
- Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Iba1 antibody
See all Iba1 primary antibodies -
Description
Goat polyclonal to Iba1 -
Host species
Goat -
Specificity
This antibody is expected to recognise P55008-1 or isoform 3 (NP_001614.3) only. For WB, we recommend blocking in 3-5% milk, NOT BSA. Blocking in BSA gives high background. Primary incubation 1 hour at room temperature.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P MouseWB Human
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Immunogen
Synthetic peptide corresponding to Human Iba1 aa 28-42 (internal sequence) (Cysteine residue). NP_001614.3
Sequence:C-NKQFLDDPKYSSDED
Database link: P55008-1 -
Positive control
- WB: Human Frontal Cortex lysate. IHC-P: Mouse brain. ICC/IF: Caco-2 cells Flow Cyt: K562 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody (ab48004)
Immunocytochemistry/ Immunofluorescence analysis of Caco-2 cells labeling Iba1 with ab48004 at 10µg/mL. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 secondary antibody at 2µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
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Western blot - Anti-Iba1 antibody (ab48004)Anti-Iba1 antibody (ab48004) at 0.5 µg/ml + human frontal cortex lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 17 kDaPrimary incubation was 1 hour
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab48004)Paraffin embedded mouse brain tissue stained for Iba1 with ab48004 (2 µg/ml) in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab48004) This image is courtesy of an Abreview submitted by Mr Carl Hobbsab48004 at 1/300 staining human jejunum tissue sections by IHC-P. A heat mediated antigen retrieval step in citric acid buffer was performed and the tissue was blocked in BSA before incubation with the antibody for 2 hours. A biotinylated rabbit anti-goat antibody was used as the secondary. -
Flow Cytometry - Anti-Iba1 antibody (ab48004)Flow Cytometry analysis of K562 cells labeling Iba1 with ab48004 at 10µg/mL (blue). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton. An Alexa Fluor 488 secondary antibody at 1µg/mL was used as the secondary antibody.
IgG control: Unimmunized goat IgG (black) followed by Alexa Fluor 488 secondary antibody.
