Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - BSA and Azide free (ab250900)
![Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - BSA and Azide free (ab250900)](https://www.abcam.com/ps/products/250/ab250900/Images/ab250900-406351-anti-hydrogen-potassium-atpase-beta-atp4b-antibody-epr8819-2-bsa-and-azide-free-western-blot.jpg "Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - BSA and Azide free (ab250900)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8819(2)] to Hydrogen Potassium ATPase Beta/ATP4B - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
-
Product name
Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - BSA and Azide free
See all Hydrogen Potassium ATPase Beta/ATP4B primary antibodies -
Description
Rabbit monoclonal [EPR8819(2)] to Hydrogen Potassium ATPase Beta/ATP4B - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab250900 is the carrier-free version of ab186749 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250900 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Hydrogen Potassium ATPase Beta
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR8819(2) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - BSA and Azide free (ab250900)Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749) at 1/1000 dilution + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDaThis data was developed using ab186749, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - BSA and Azide free (ab250900)](https://www.abcam.com/ps/products/250/ab250900/Images/ab250900-403231-anti-hydrogen-potassium-atpase-beta-atp4b-antibody-epr8819-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - BSA and Azide free (ab250900)Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749) at 1/5000 dilution + Human stomach lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 33 kDaThis data was developed using ab186749, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - BSA and Azide free (ab250900)](https://www.abcam.com/ps/products/250/ab250900/Images/ab250900-399379-anti-hydrogen-potassium-atpase-beta-atp4b-antibody-epr8819-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - BSA and Azide free (ab250900)Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749) at 1/1000 dilution + Human Hydrogen Potassium ATPase Beta/ATP4B recombinant full length protein with proprietary tag at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDaThis data was developed using ab186749, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - BSA and Azide free (ab250900)](https://www.abcam.com/ps/products/250/ab250900/Images/ab250900-4-benefits-of-recombinant-antibodies.png)
Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - BSA and Azide free (ab250900)