Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5539-71-8] to Human Kappa Chain - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free
    See all Human Kappa Chain primary antibodies

  • Description

    Rabbit monoclonal [EPR5539-71-8] to Human Kappa Chain - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Other Immunogen Type. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human tonsil, Human spleen, Human thymus, and Human plasma lysates. B cell lymphoma tissue. Human tonsil tissue. ICC: Raji cells

  • General notes

    ab211641 is the carrier-free version of ab134929 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab211641 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5539-71-8

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
    Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
    All lanes : Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929) at 1/1000 dilution

    Lane 1 : Human tonsil tissue lysate
    Lane 2 : Human spleen tissue lysate
    Lane 3 : Human thymus tissue lysate
    Lane 4 : Human plasma

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution

    Predicted band size: 49 kDa



    This data was developed using ab134929, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
    This data was developed using ab134929, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded B cell lymphoma tissue labelling IgM with ab134929 at 1/250 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
    Immunocytochemistry - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)

    This data was developed using the same antibody clone in a different buffer formulation (ab134929).
    Immunocytochemistry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) labeling Human Kappa Chain with purified ab134929 at 1/100 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.32 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)

    Clone EPR5539-71-8 (ab211641) has been successfully conjugated by Abcam. This image was generated using Anti-Human Kappa Chain antibody [EPR5539-71-8] (Alexa Fluor® 647). Please refer to ab200631 for protocol details.

    IHC image of human kappa chain staining in a section of formalin-fixed paraffin-embedded normal human tonsil*.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab200631 at 1/50 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
    This data was developed using ab134929, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling IgM with ab134929 at 1/250 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
    This data was developed using ab134929, the same antibody clone in a different buffer formulation.Immunohistochemical analysis using ab134929 showing positive staining in Normal colon, lymphocytes tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
    This data was developed using ab134929, the same antibody clone in a different buffer formulation.Immunohistochemical analysis using ab134929 showing positive staining in Normal stomach, lymphocytes tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
    Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)

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