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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-Hsp70 (phospho S153) antibody (ab228421)

Anti-Hsp70 (phospho S153) antibody (ab228421)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Hsp70 (phospho S153)
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Hsp70 (phospho S153) antibody
    See all Hsp70 primary antibodies
  • Description

    Rabbit polyclonal to Hsp70 (phospho S153)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Hsp70 (phospho S153).
    Database link: P0DMV8

  • Positive control

    • WB: T47D treated with EGF, cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.50
    Constituents: 0.24% HEPES, 0.87% Sodium chloride, 50% Glycerol, 0.01% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab228421 is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
  • Clonality

    Polyclonal
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cancer
    • Tumor biomarkers
    • Other

Images

  • Western blot - Anti-Hsp70 (phospho S153) antibody (ab228421)
    Western blot - Anti-Hsp70 (phospho S153) antibody (ab228421)
    All lanes : Anti-Hsp70 (phospho S153) antibody (ab228421) at 1/1000 dilution

    Lane 1 : T-47D (human ductal breast epithelial tumor cell line) cell lysate treated with 1 nM EGF for 60 minutes
    Lane 2 : T-47D (human ductal breast epithelial tumor cell line) cell lysate treated with 1 nM EGF for 60 minutes, plus lambda phosphatase (1200 units for 30 minutes)

    Developed using the ECL technique.

    Predicted band size: 70 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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