Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4576] to Hsp105/HSP110 - BSA and Azide free
  • Suitable for: ICC, IHC-P, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free
    See all Hsp105/HSP110 primary antibodies

  • Description

    Rabbit monoclonal [EPR4576] to Hsp105/HSP110 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247912 is the carrier-free version of ab109624 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247912 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Hsp105

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4576

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)
    Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)
    All lanes : Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624) at 1/5000 dilution (purified)

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 97 kDa
    Observed band size: 105 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab109624, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)
    Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)
    All lanes : Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624) at 1/5000 dilution (purified)

    Lane 1 : C6 whole cell lysate
    Lane 2 : PC-12 whole cell lysate
    Lane 3 : NIH/3T3 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 97 kDa
    Observed band size: 105 kDa why is the actual band size different from the predicted?



    This data was developed using ab109624, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)

    This data was developed using ab109624, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue labelling Hsp105/HSP110 with purified ab109624 at a dilution of 1/500. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  • Immunocytochemistry - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)
    Immunocytochemistry - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)
    This data was developed using ab109624, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Hsp105/HSP110 with purified ab109624 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used. Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000). Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
  • Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)
    Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)

    This data was developed using ab109624&

    44; the same antibody clone in a different buffer formulation.

    >ab109624 (purified) at 1/40 immunoprecipitating Hsp105/HSP110 in NIH/3T3 whole cell lysate.

    Lane 1 (input): NIH/3T3 whole cell lysate (10µg)

    Lane 2 (+): ab109624 + NIH/3T3 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109624 in NIH/3T3 whole cell lysate.

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)
    Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)
    All lanes : Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Human cerebellum lysate
    Lane 4 : MCF-7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 97 kDa



    This data was developed using ab109624, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)
    Immunocytochemistry - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)
    This data was developed using ab109624, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Hsp105/HSP110 with unpurified ab109624 at a dilution of 1/100.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)
    This data was developed using ab109624, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid gland carcinoma tissue labelling Hsp105/HSP110 with unpurified ab109624 at a dilution of 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)
    Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (ab247912)

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