Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HRG antibody [EPR24184-52] (ab281563)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24184-52] to HRG
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HRG antibody [EPR24184-52]
    See all HRG primary antibodies

  • Description

    Rabbit monoclonal [EPR24184-52] to HRG

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt (Intra),ICC or IHC-Fr

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human HRG aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P04196

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Human plasma and liver tissue lysate. IHC-P: Human liver and colon tissue lysate. IP: Human liver tissue lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. 

    For details on our patents, please refer to www.abcam.com/RabMAbPatents

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24184-52

  • Isotype

    IgG

Images

  • Western blot - Anti-HRG antibody [EPR24184-52] (ab281563)
    Western blot - Anti-HRG antibody [EPR24184-52] (ab281563)
    All lanes : Anti-HRG antibody [EPR24184-52] (ab281563) at 1/1000 dilution

    Lane 1 : Human plasma at 20 µl
    Lane 2 : Human liver tissue lysate at 20 µg
    Lane 3 : Human pancreas tissue lysate at 20 µg

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
    Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 60 kDa



    The MW and expression pattern is consistent to previous referecence (PMID:14744774, PMID:25363753, PMID:19712047)
    Low expression or negative: human pancreas (PMID:25363753)

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lane 1: 3.25 seconds; 

    Lane 2-3: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRG antibody [EPR24184-52] (ab281563)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRG antibody [EPR24184-52] (ab281563)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HRG with ab281563 at 1/5000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human liver. (PMID: 21215706) is observed. The section was incubated with ab281563 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

    Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRG antibody [EPR24184-52] (ab281563)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRG antibody [EPR24184-52] (ab281563)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HRG with ab281563 at 1/5000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the plasma and stroma cells in human colon (PMID: 25243896) is observed. The section was incubated with ab281563 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

    Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunoprecipitation - Anti-HRG antibody [EPR24184-52] (ab281563)
    Immunoprecipitation - Anti-HRG antibody [EPR24184-52] (ab281563)

    HRG was immunoprecipitated from 0.35 mg of Human liver tissue lysate with ab281563 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab281563 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366), was used as secondary antibody at 1/5000 dilution.


    Lane 1: Human liver tissue lysate 10µg (Input).
    Lane 2: ab281563 IP in Human liver tissue lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab281563 in Human liver tissue lysate.


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 15 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRG antibody [EPR24184-52] (ab281563)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRG antibody [EPR24184-52] (ab281563)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling HRG with ab281563 at 1/5000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining on human tonsil. The section was incubated with ab281563 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

    Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRG antibody [EPR24184-52] (ab281563)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRG antibody [EPR24184-52] (ab281563)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling HRG with ab281563 at 1/5000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining on human pancreas. The section was incubated with ab281563 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

    Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-HRG antibody [EPR24184-52] (ab281563)
    Anti-HRG antibody [EPR24184-52] (ab281563)

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