Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

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Anti-HLA DMB antibody [EPR7982] (ab131273)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7982] to HLA DMB
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-HLA DMB antibody [EPR7982]
See all HLA DMB primary antibodies
Description

Rabbit monoclonal [EPR7982] to HLA DMB
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide corresponding to residues at the C terminus of Human HLA DMB (UniProt P28068).
Positive control
- Raji and Daudi cell lysates. Human colon tissue and tonsil tissue. Raji cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR7982
Isotype

IgG
Research areas

Western blot - Anti-HLA DMB antibody [EPR7982] (ab131273)

All lanes : Anti-HLA DMB antibody [EPR7982] (ab131273) at 1/1000 dilution

Lane 1 : Raji cell lysate
Lane 2 : Daudi cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DMB antibody [EPR7982] (ab131273)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling HLA DMB with ab131273 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DMB antibody [EPR7982] (ab131273)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling HLA DMB with ab131273 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-HLA DMB antibody [EPR7982] (ab131273)

Immunofluorescence analysis of Raji cells labelling HLA DMB with ab131273 at 1/250 dilution.
Anti-HLA DMB antibody [EPR7982] (ab131273)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com