Anti-Histone H4 (symmetric di methyl R3) antibody (ab231658)
Key features and details
- Rabbit polyclonal to Histone H4 (symmetric di methyl R3)
- Suitable for: ICC/IF, Dot blot, WB
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-Histone H4 (symmetric di methyl R3) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (symmetric di methyl R3) -
Host species
Rabbit -
Specificity
ab231658 also recognizes H2AR3me2(sym). -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB HumanRecombinant fragment
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Immunogen
Synthetic peptide corresponding to Human Histone H4 (symmetric di methyl R3) conjugated to keyhole limpet haemocyanin.
Database link: P62805 -
Positive control
- ICC/IF: HeLa cells. WB: Whole cell and histone extracts from HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal
Images
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Western blot - Anti-Histone H4 (symmetric di methyl R3) antibody (ab231658)All lanes : Anti-Histone H4 (symmetric di methyl R3) antibody (ab231658) at 1/1000 dilution
Lane 1 : Whole cell HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts at 25 µg
Lane 2 : Histone extracts from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells at 15 µg
Lane 3 : Recombinant histone H2A at 1 µg
Lane 4 : Recombinant histone H2B at 1 µg
Lane 5 : Recombinant histone H3 at 1 µg
Lane 6 : Recombinant histone H4 at 1 µgDilution buffer: TBS-Tween containing 5% skimmed milk.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (symmetric di methyl R3) antibody (ab231658)HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (symmetric di methyl R3) using ab231658 at a dilution of 1/500 in ICC/IF.
Cells were fxed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Secondary used is an Alexa Fluor®488-conjugated anti-Rabbit IgG. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
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Dot Blot - Anti-Histone H4 (symmetric di methyl R3) antibody (ab231658)Dot Blot analysis was performed with peptides containing other histone arginine methylations and the unmodifed H4R3. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. ab231658 was used at a dilution of 1:5,000.
