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Anti-Histone H4 (mutated G48D) antibody [EP19111] (ab199739)

Price and availability

301 536 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Histone H4 (mutated G48D) antibody [EP19111] (ab199739)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP19111] to Histone H4 (mutated G48D)
  • Suitable for: WB, PepArr
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Histone H4 (mutated G48D) antibody [EP19111]
  • Description

    Rabbit monoclonal [EP19111] to Histone H4 (mutated G48D)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, PepArrmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate, His-tagged human Histone H4 (mutant G48D, aa1-103) recombinant protein.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP19111
  • Isotype

    IgG

Images

  • Western blot - Anti-Histone H4 (mutated G48D) antibody [EP19111] (ab199739)
    Western blot - Anti-Histone H4 (mutated G48D) antibody [EP19111] (ab199739)
    All lanes : Anti-Histone H4 (mutated G48D) antibody [EP19111] (ab199739) at 1/1000 dilution

    Lane 1 : His-tagged human Histone H4 (mutant G48D, aa1-103) recombinant protein, 20 ng
    Lane 2 : His-tagged human Histone H4 (WT, aa1-103) recombinant protein, 20 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    Blocking/Dilution buffer: 5% BSA/TBST.

    Exposure times: Lane 1: 10 seconds; Lane 2: 3 minutes.

  • Western blot - Anti-Histone H4 (mutated G48D) antibody [EP19111] (ab199739)
    Western blot - Anti-Histone H4 (mutated G48D) antibody [EP19111] (ab199739)
    All lanes : Anti-Histone H4 (mutated G48D) antibody [EP19111] (ab199739) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) cytoplasm lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) nuclear lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 1 second


    Blocking/Diluting buffer and concentration 5% BSA/TBST

  • Peptide Array - Anti-Histone H4 (mutated G48D) antibody [EP19111] (ab199739)
    Peptide Array - Anti-Histone H4 (mutated G48D) antibody [EP19111] (ab199739)

    All batches of ab199739 are tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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