Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Histone H4 (mono methyl R78) antibody [EPR19561] (ab202036)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19561] to Histone H4 (mono methyl R78)
Suitable for: Dot blot, ICC/IF, Flow Cyt, WB
Reacts with: Mouse, Human

Product name

Anti-Histone H4 (mono methyl R78) antibody [EPR19561]
See all Histone H4 primary antibodies
Description

Rabbit monoclonal [EPR19561] to Histone H4 (mono methyl R78)
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa and NIH/3T3 whole cell lysates. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. Dot blot: Histone H4 (mono methyl R78) peptide.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19561
Isotype

IgG
Research areas

Western blot - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] (ab202036)

All lanes : Anti-Histone H4 (mono methyl R78) antibody [EPR19561] (ab202036) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma ) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 11 kDa
Observed band size: 11 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Dot Blot - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] (ab202036)

Dot blot analysis of Histone H4 (mono methyl R78) labeled with ab202036 at 1/1000 dilution.

Lane 1: Histone H4 (mono methyl R78) peptide.

Lane 2: Histone H4 (asymmetric di methyl R78) peptide.

Lane 3: Histone H4 (symmetric di methyl R78) peptide.

Lane 4: Histone H4 unmodified peptide.

Lane 5: Histone H4 (mono methyl K77) peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Exposure time: 3 minutes.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] (ab202036)

Immunofluorescent analysis of 2% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H4 (mono methyl R78) with ab202036 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on Hela cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] (ab202036)

Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Histone H4 (mono methyl R78) with ab202036 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Anti-Histone H4 (mono methyl R78) antibody [EPR19561] (ab202036)

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