Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H4 (methyl R67) antibody [EPR18914] - BSA and Azide free (ab250000)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18914] to Histone H4 (methyl R67) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Histone H4 (methyl R67) antibody [EPR18914] - BSA and Azide free
    See all Histone H4 primary antibodies

  • Description

    Rabbit monoclonal [EPR18914] to Histone H4 (methyl R67) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects mono methyl, asymmetric di methyl and symmetric di methyl arginine 67.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250000 is the carrier-free version of ab177827. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250000 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18914

  • Isotype

    IgG

Images

  • Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] - BSA and Azide free (ab250000)
    Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] - BSA and Azide free (ab250000)
    Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827) at 1/5000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 1 second


    This data was developed using ab177827, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] - BSA and Azide free (ab250000)
    Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] - BSA and Azide free (ab250000)
    All lanes : Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827) at 1/5000 dilution

    Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 15 seconds


    This data was developed using ab177827, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] - BSA and Azide free (ab250000)
    Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] - BSA and Azide free (ab250000)
    All lanes : Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cells) whole cell lysate
    Lane 3 : Mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    This data was developed using ab177827, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-2: 4 seconds; Lane 3: 15 seconds.

  • Anti-Histone H4 (methyl R67) antibody [EPR18914] - BSA and Azide free (ab250000)
    Anti-Histone H4 (methyl R67) antibody [EPR18914] - BSA and Azide free (ab250000)

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