Human Caveolin-2 ELISA Kit (CAV2) (ab270881)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 38.91 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Caveolin-2 ELISA Kit (CAV2)
See all Caveolin-2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 4% -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
38.91 pg/ml -
Range
78.13 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 115 % - % Cell Lysate 102 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Caveolin-2 ELISA kit (ab270881) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Caveolin-2 protein in human cell and tissue samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Caveolin-2 with 38.91 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
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Notes
Caveolin-2 is a scaffolding protein found in the inner surface of caveolae. CAV-2 and CAV-1 form a hetero-oligomeric complex involved in signal transduction, apoptosis, cell growth and lipid metabolism. CAV-2 and CAV-1 expression in breast cancer is associated with basal-like and triple-negative immunophenotype.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Caveolin-2 (CAV2) Capture Antibody 1 x 600µl 10X Human Caveolin-2 (CAV2) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human Caveolin-2 (CAV2) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression. -
Tissue specificity
Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts. -
Sequence similarities
Belongs to the caveolin family. -
Post-translational
modificationsPhosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin. -
Cellular localization
Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments. - Information by UniProt
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Alternative names
- CAV
- CAV2
- CAV2_HUMAN
see all -
Database links
- Entrez Gene: 858 Human
- Omim: 601048 Human
- SwissProt: P51636 Human
- Unigene: 212332 Human
- Unigene: 603096 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Caveolin-2 standard curve in 1X Cell Extraction Buffer PTR.The Caveolin-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Caveolin-2 in human A549 and heart tissue homogenate extract based on a 500 µg/mL and 250 µg/mL extract loads, respectively.The concentrations of Caveolin-2 were measured in duplicate and interpolated from the Caveolin-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Caveolin-2 concentration was determined to be 1,974.86 pg/mL in A549 extract and 3,873.07 pg/mL in heart homogenate extract.
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Sandwich ELISA - Human Caveolin-2 ELISA Kit (CAV2) (ab270881)To learn more about the advantages of recombinant antibodies see here.
