Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)

Key features and details

  • Mouse monoclonal [mAbcam 17036] to Histone H4 - ChIP Grade
  • Suitable for: IHC-P, ChIP, WB
  • Reacts with: Mouse, Rat, Cow, Human, Recombinant fragment
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade
    See all Histone H4 primary antibodies

  • Description

    Mouse monoclonal [mAbcam 17036] to Histone H4 - ChIP Grade

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    IHC-P
    Human
    WB
    Mouse
    Cow
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Histone H4 aa 50 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: P62805
    (Peptide available as ab13843)

  • Positive control

    • This antibody gave a positive signal in the following: Calf thymus histone preparation; HeLa histone preparation; Histone H4 recombinant protein; NIH3T3 Whole Cell Lysate; MEF1 Whole Cell Lysate; PC12 Whole Cell Lysate.

  • General notes

    This antibody clone is manufactured by Abcam.

    To detect histone H4 by ICC/IF, we recommend using ab177840 or ab9052 since these antibodies perform better in this application.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    IgG fraction

  • Clonality

    Monoclonal

  • Clone number

    mAbcam 17036

  • Myeloma

    Sp2/0-Ag14

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

Images

  • ChIP - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    ChIP - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab17036 (blue), and 20µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    IHC image of ab17036 staining in Human Hodgekin's Lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17036, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    All lanes : Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036) at 2 µg/ml

    Lane 1 : Histone H1 Recombinant Protein
    Lane 2 : Histone H2A Recombinant Protein
    Lane 3 : Histone H2B Recombinant Protein
    Lane 4 : Histone H3.1 Recombinant Protein
    Lane 5 : Histone H4 Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 30 seconds
  • Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    All lanes : Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute
  • Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    All lanes : Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036) at 5 µg/ml

    Lane 1 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
    Lane 2 : HeLa Histone Preparation Nuclear Lysate at 2.5 µg

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)

    IHC image of Histone H4 staining in formalin fixed, paraffin embedded human Hodgkin's lymphoma tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17036, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)
    All lanes : Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036) at 5 µg/ml

    Lane 1 : Histone H1 Recombinant Protein
    Lane 2 : Histone H2A Recombinant Protein
    Lane 3 : Histone H2B Recombinant Protein
    Lane 4 : Histone H3.1 Recombinant Protein
    Lane 5 : Histone H4 Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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