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Anti-Histone H3 antibody [1B1B2] - ChIP Grade (ab195277)

Key features and details

  • Mouse monoclonal [1B1B2] to Histone H3 - ChIP Grade
  • Suitable for: ICC/IF, WB, ChIP
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG3

Overview

  • Product name

    Anti-Histone H3 antibody [1B1B2] - ChIP Grade
    See all Histone H3 primary antibodies

  • Description

    Mouse monoclonal [1B1B2] to Histone H3 - ChIP Grade

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Histone H3 (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: P68431

  • Positive control

    • HeLa, K562, MCF7, U2OS, HepG2, Jurkat, NIH 3T3 and E14Tg2a mouse ES whole cell extracts.

  • General notes

    ab195277 can be used as a loading control for ChIP and Western blot experiments

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    1B1B2

  • Isotype

    IgG3

  • Research areas

Images

  • ChIP - Anti-Histone H3 antibody [1B1B2] - ChIP Grade (ab195277)
    ChIP - Anti-Histone H3 antibody [1B1B2] - ChIP Grade (ab195277)

    ChIP assays were performed using human HeLa cells, ab195277 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the active GAPDH and EIF4A2 genes, and for the inactive MYOD1 gene and the Sat2 satellite repeat. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

  • Western blot - Anti-Histone H3 antibody [1B1B2] - ChIP Grade (ab195277)
    Western blot - Anti-Histone H3 antibody [1B1B2] - ChIP Grade (ab195277)
    All lanes : Anti-Histone H3 antibody [1B1B2] - ChIP Grade (ab195277) at 1/5000 dilution

    Lane 1 : HeLa cell lysates at 40 µg
    Lane 2 : Recombinant histone H3 at 1 µg
    Lane 3 : Recombinant histone H4 at 1 µg

    Predicted band size: 15 kDa

  • Western blot - Anti-Histone H3 antibody [1B1B2] - ChIP Grade (ab195277)
    Western blot - Anti-Histone H3 antibody [1B1B2] - ChIP Grade (ab195277)
    All lanes : Anti-Histone H3 antibody [1B1B2] - ChIP Grade (ab195277) at 1/1000 dilution

    Lane 1 : HeLa whole cell extract
    Lane 2 : K562 whole cell extract
    Lane 3 : MCF7 whole cell extract
    Lane 4 : U2OS whole cell extract
    Lane 5 : HepG2 whole cell extract
    Lane 6 : Jurkat whole cell extract
    Lane 7 : NIH 3T3 whole cell extract
    Lane 8 : E14Tg2a Mouse ES whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 15 kDa



    The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody [1B1B2] - ChIP Grade (ab195277)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody [1B1B2] - ChIP Grade (ab195277)

    Immunofluorescent analysis of HeLa cells labeling Histone H3 with ab195277 at 1/500 dilution. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 1% BSA. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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