Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Thursday March 04, 2021

Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)

Key features and details

Mouse monoclonal [OTI7A4] to alpha Actinin/ACTN1
Suitable for: WB, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human, African green monkey
Isotype: IgG2a

Product name

Anti-alpha Actinin/ACTN1 antibody [OTI7A4]
See all alpha Actinin/ACTN1 primary antibodies
Description

Mouse monoclonal [OTI7A4] to alpha Actinin/ACTN1
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human alpha Actinin/ACTN1 aa 650-893. Protein produced in E.coli.
Database link: NP_001093
Positive control
- ICC/IF: HeLa cells transiently transfected by pCMV6-ENTRY alpha Actinin/ATCN1. HeLa cells. WB: HEK-293T cell transient overexpression lysate, HepG, NIH/3T3, HeLa, SVT2, A549, COS-7, Jurkat, PC-12, and MCF7 cell extracts. Mouse spleen tissue extracts. IHC-P: Human breast, kidney, liver, ovary and bladder tissue.
General notes

The clone number has been updated from 7A4 to OTI7A4, both clone numbers name the same clone.
This product was previously labelled as alpha Actinin

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography
Clonality

Monoclonal
Clone number

OTI7A4
Isotype

IgG2a
Research areas

Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)

All lanes : Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421) at 1/200 dilution

Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts
Lane 3 : SVT2 (Mouse cell line) extracts
Lane 4 : A549 (Human lung carcinoma cell line) extracts
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) extracts
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) extracts
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) extracts
Lane 8 : MCF7 (Human breast adenocarcinoma cell line) extracts

Lysates/proteins at 10 µg per lane.

Predicted band size: 103 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)

Paraffin-embedded human breast tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for alpha Actinin/ATCN1 using ab90421 at a 1/100 dilution in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)

Paraffin-embedded human kidney tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)

Paraffin-embedded human bladder tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)

Paraffin-embedded human ovary tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)

Paraffin-embedded human liver tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)

pCMV6-ENTRY alpha Actinin/ATCN1 cDNA transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for alpha Actinin/ATCN1 using ab90421 at a 1/100 dilution in ICC/IF.
Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)

All lanes : Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421) at 1/200 dilution

Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) extracts
Lane 2 : Mouse brain tissue extracts
Lane 3 : Mouse liver tissue extracts
Lane 4 : Mouse spleen tissue extracts

Lysates/proteins at 10 µg per lane.

Predicted band size: 103 kDa
Western blot - Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)

All lanes : Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421) at 1/2000 dilution

Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were transfected with the pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells were transfected with pCMV6-ENTRY alpha Actinin/ATCN1
cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 103 kDa

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