Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)
![Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)](https://www.abcam.com/ps/products/213/ab213291/Images/ab213291-280555-anti-histone-h4-antibody-igx4696h-chip-grade-western-blot.jpg "Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [IGX4696H] to Histone H4 - ChIP Grade
- Suitable for: WB, ChIP
- Reacts with: Mouse, Cow, Human, Recombinant fragment
Overview
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Product name
Anti-Histone H4 antibody [IGX4696H] - ChIP Grade
See all Histone H4 primary antibodies -
Description
Human monoclonal [IGX4696H] to Histone H4 - ChIP Grade -
Host species
Human -
Tested Applications & Species
See all applications and species dataApplication Species ChIP MouseHumanWB MouseCowHumanRecombinant fragment
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: CTH, HeLa whole cell lysate, HeLa nuclear lysate, NIH3T3 whole cell lysate, NIH3T3 nuclear lysate and Histone H4B Recombinant Protein. ChIP: HeLa and NIH3T3.
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General notes
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant antibody.
Human monoclonal antibody.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
IGX4696H -
Isotype
IgG1
Images
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Western blot - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)All lanes : Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291) at 0.2 µg/ml
Lane 1 : CTH (Calf Thymus Histone) at 0.5 µg
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Nuclear Lysate at 10 µg
Lane 6 : Histone H2A Recombinant Protein at 0.1 µg
Lane 7 : Histone H4 Recombinant Protein at 0.1 µg
Secondary
All lanes : HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213291 overnight at 4°C. Antibody binding was detected using an anti-human antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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![ChIP - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)](https://www.abcam.com/ps/products/213/ab213291/Images/ab213291-280554-anti-histone-h4-antibody-igx4696h-chip-grade-chip.jpg)
ChIP - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab213291 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
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![ChIP - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)](https://www.abcam.com/ps/products/213/ab213291/Images/ab213291-280553-anti-histone-h4-antibody-igx4696h-chip-grade-chip.jpg)
ChIP - Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)Chromatin was prepared from NIH3T3 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab213291 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.
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![Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)](https://www.abcam.com/ps/products/213/ab213291/Images/ab213291-4-benefits-of-recombinant-antibodies.png)
Anti-Histone H4 antibody [IGX4696H] - ChIP Grade (ab213291)
