Anti-Histone H3 (tri methyl K64) antibody (ab195483)
Key features and details
- Rabbit polyclonal to Histone H3 (tri methyl K64)
- Suitable for: ChIP, WB, Dot blot
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (tri methyl K64) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (tri methyl K64) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (tri methyl K64) conjugated to keyhole limpet haemocyanin. (peptide surrounding K64)
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Positive control
- K562 cells; histone extracts from HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-Histone H3 (tri methyl K64) antibody (ab195483)
ChIP analysis using K562 cells, ab195483 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the Sat2 and Sata satellite repeats, used as positive controls.
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Dot Blot - Anti-Histone H3 (tri methyl K64) antibody (ab195483)Dot Blot analysis was performed to test the cross reactivity of ab195483 against Histone H3 (tri methyl K64) (H3K64me3) with peptides containing different modifications of histone H3 and H4 or the unmodified H3K64 sequence. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. ab195483 was used at 1/5,000 dilution.
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Western blot - Anti-Histone H3 (tri methyl K64) antibody (ab195483)Anti-Histone H3 (tri methyl K64) antibody (ab195483) at 1/100 dilution + histone extracts from HeLa cells at 30 µg
Predicted band size: 15 kDaThe antibody was diluted 1/100 in TBS-Tween containing 5% skimmed milk.
