Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade (ab176844)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17704] to Histone H3 (mono methyl R2) - ChIP Grade
  • Suitable for: ChIP, PepArr, WB, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade
    See all Histone H3 primary antibodies

  • Description

    Rabbit monoclonal [EPR17704] to Histone H3 (mono methyl R2) - ChIP Grade

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    ICC/IF
    Human
    PepArr
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3 and HeLa whole cell lysates. ICC/IF: HeLa cells. ChIP: Chromatin from HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ChIP - Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade (ab176844)
    ChIP - Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade (ab176844)

    Chromatin was prepared from HeLa (Human epithelial cells from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab176844 (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.

  • Western blot - Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade (ab176844)
    Western blot - Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade (ab176844)
    Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade (ab176844) at 1/5000 dilution + NIH/3T3 (mouse embryo) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5%BSA /TBST.

  • Western blot - Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade (ab176844)
    Western blot - Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade (ab176844)
    Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade (ab176844) at 1/5000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5%BSA /TBST.

  • Peptide Array - Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade (ab176844)
    Peptide Array - Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade (ab176844)

    ab176884 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade (ab176844)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade (ab176844)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H3 (mono methyl R2) with ab176844 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab176844 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade (ab176844)
    Anti-Histone H3 (mono methyl R2) antibody [EPR17704] - ChIP Grade (ab176844)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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