Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-18BP antibody [EP1088Y] (ab52914)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1088Y] to IL-18BP
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-IL-18BP antibody [EP1088Y]
    See all IL-18BP primary antibodies

  • Description

    Rabbit monoclonal [EP1088Y] to IL-18BP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human IL-18BP aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O95998

  • Positive control

    • WB: HeLa and K562 cell lysates and rat kidney and heart tissue lysates. IHC-P: human tonsil tissue.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)
    Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)
    All lanes : Anti-IL-18BP antibody [EP1088Y] (ab52914) at 1/2000 dilution (purified)

    Lane 1 : Rat kidney tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 22 kDa
    Observed band size: 44 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 1% BSA/TBST.

  • Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)
    Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)
    All lanes : Anti-IL-18BP antibody [EP1088Y] (ab52914) at 1/1000 dilution (purified)

    Lane 1 : HeLa whole cell lysate
    Lane 2 : K562 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 22 kDa
    Observed band size: 44 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 1% BSA/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18BP antibody [EP1088Y] (ab52914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18BP antibody [EP1088Y] (ab52914)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IL-18BP with purified ab52914 at 1/400. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)
    Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)
    Anti-IL-18BP antibody [EP1088Y] (ab52914) at 1/500 dilution (unpurified) + HeLa cell lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 22 kDa
    Observed band size: 44 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18BP antibody [EP1088Y] (ab52914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18BP antibody [EP1088Y] (ab52914)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IL-18BP with unpurified ab52914 at a dilution of 1/50.

  • Anti-IL-18BP antibody [EP1088Y] (ab52914)
    Anti-IL-18BP antibody [EP1088Y] (ab52914)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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