Human IL1B knockout THP-1 cell lysate (ab275508)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab277322 - Human IL-1B knockout THP-1 cell lysate - LPS treated 1 x 100µg ab277323 - Human IL-1B knockout THP-1 cell lysate 1 x 100µg ab277324 - Human wild-type THP-1 cell lysate - LPS treated 1 x 100µg ab277314 - Human wild-type THP-1 cell lysate 1 x 100µg -
Research areas
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Cell type
acute monocytic leukemia -
Disease
Acute Monocytic Leukemia -
Gender
Male
Images
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Western blot - Human IL1B knockout THP-1 cell lysate (ab275508)
Lane 1: Wild-type THP-1 untreated cell lysate 30 μg
Lane 2: Wild-type THP-1 LPS-treated (3 h, 100 ng/ml) cell lysate 30 μg
Lane 3: IL1B knockout THP-1 untreated cell lysate 30 μg
Lane 4: IL1B knockout THP-1 LPS-treated (3 h, 100 ng/ml) cell lysate 30 μg
Lanes 1 - 4:Merged signal (red and green). Green - ab216995 observed at 27-32 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab216995 was shown to react with IL-1 beta in wild-type THP-1 cells in Western blot with loss of signal observed in IL1B knockout cell line ab273762 (knockout cell lysate ab275508). Wild-type THP-1 and IL1B knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab216995 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging. -
Sanger Sequencing - Human IL1B knockout THP-1 cell lysateAllele-1: 47 bp deletion in exon 4.