Human IL-2 ELISA Kit (ab270883)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 32.1 pg/ml
- Range: 39 pg/ml - 2500 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-2 ELISA Kit
See all IL-2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 3.2% Inter-assay Sample n Mean SD CV% Supernatant 4 8.7% -
Sample type
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
32.1 pg/ml -
Range
39 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 108 100% - 120% Serum 86 83% - 88% Hep Plasma 82 80% - 85% EDTA Plasma 89 86% - 93% Cit plasma 87 84% - 91% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-2 ELISA kit (ab270883) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-2 protein in human serum, plasmas and cell and tissue supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human IL-2 with 32.1 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY: This kit recognizes both native and recombinant human IL-2 protein in stimulated serum, plasmas, and cell culture supernatant.
Urine, milk, saliva, and cell and tissue extract samples have not been tested with this kit.
CROSS REACTIVITY: Recombinant human IL-2R alpha was prepared at 5,000 pg/mL and assayed for cross reactivity. No cross-reactivity was observed.
INTERFERENCE: Recombinant human IL-2R alpha was prepared at 5,000 pg/mL and tested for interference. No interference with was observed.
SPECIES REACTIVITY: This kit recognizes human IL-2 protein.
No signal was observed in 50% serum samples from the following species: Mouse, Rat, Cow.
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Notes
IL-2, also known as T cell growth factor (TCGF), is a glycosylated alpha-helical polypeptide, synthesized as a 153 amino acid (aa) precursor with a 20 aa signal peptide and a 133 aa mature chain. It is secreted by activated CD4+ and CD8+ T cells, neurons, microglia and hematopoietic stem cells in response to antigenic or mitogenic stimulation. IL-2 is required for T-cell proliferation, Natural Killer cells (NK) cytolytic activity, differentiation of regulatory T cells, modulation of T helper (Th) cell differentiation and activation-induced cell death. IL-2 modulates the expression of receptors for other cytokines and transcription factors, therefore regulating cytokine cascades that correlate with each of the Th differentiation states. IL-2 is both an immune stimulator and immune suppressor cytokine, which efficiently controls the immune system to deal with autoimmunity and adaptive immune response.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-2 Capture Antibody 1 x 600µl 10X Human IL-2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human IL-2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Aldesleukin
- IL 2
- IL-2
see all -
Database links
- Entrez Gene: 3558 Human
- Omim: 147680 Human
- SwissProt: P60568 Human
- Unigene: 89679 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human IL-2 standard curve comparisonStandard Curve comparison between the original Human IL-2 SimpleStep ELISA kit (ab174444) and current Human IL-2 SimpleStep ELISA kit (ab270883).
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Example of human IL-2 standard curve in Sample Diluent NS.The IL-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Standard curve in Sample Diluent NSExample of human IL-2 standard curve in Sample Diluent NS. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Standard curve in Sample Diluent 50BPExample of human IL-2 standard curve in Sample Diluent 50BP. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Standard curve in Sample Diluent 50BPExample of human IL-2 standard curve in Sample Diluent 50BP. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-2 in human stimulated PBMCs.The concentrations of IL-2 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMCs stimulated with 1.5% PHAM for 48 hours (25%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 5,330 pg/mL in stimulated PBMCs.
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Assay sensitivity.The MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Recombinant IL-2 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent 50BP.
50% pooled serum samples from eight healthy donors was measured in duplicate. All values were below the detectable range of the assay.
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Linearity of dilution.Recombinant IL-2 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
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Linearity of dilution.Native IL-2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in NS.