Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab195391)
Key features and details
- Rabbit polyclonal to Histone H3 (mono methyl K4) - ChIP Grade
- Suitable for: WB, ChIP, ICC/IF, Dot blot, ChIP-sequencing
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (mono methyl K4) - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: WB, ChIP, ICC/IF, Dot blot, ChIP-sequencingmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Mouse, a wide range of other species
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Immunogen
Synthetic peptide within Human Histone H3 (mono methyl K4) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. Peptide surrounding K4.
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Positive control
- WB: HeLa whole cell extract and Histone extract. ICC/IF: HeLa cells. ChIP and ChIP seq: chromatin from K562 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP-sequencing - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab195391)
ChIP was performed on sheared chromatin from 100,000 K562 cells using 1 µg of ab195391. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Image A and B shows the H3K4me1 signal in two genomic regions containing the ACTB and GAS2L1 positive controls. The position of the amplicon used for ChIP-qPCR is indicated by an arrow. Image C shows the H3K4me1 peak distribution along a 1 Mb genomic region of chromosome 5.
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ChIP - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab195391)ChIP assays were performed using human K562 cells, ab195391 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (1 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for a region surrounding the ACTB and GAS2L1 gene, respectively, used as positive controls, and for the promoters of the GAPDH and EIF4A2 genes, used as negative controls. Image shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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Dot Blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab195391)To test the cross reactivity of the ab195391, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified Histone H3 K4 (H3K4). One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1/5000. Image shows a high specificity of the antibody for the modification of interest.
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Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab195391)All lanes : Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab195391) at 1/500 dilution
Lane 1 : HeLa whole cell extract at 25 µg
Lane 2 : Histone extracts from HeLa cells at 15 µg
Lane 3 : Recombinant histone H2A at 1 µg
Lane 4 : Recombinant histone H2B at 1 µg
Lane 5 : Recombinant histone H3 at 1 µg
Lane 6 : Recombinant histone H4 at 1 µgTBS-Tween containing 5% skimmed milk.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab195391)Immunocytochemistry/ Immunofluorescence of HeLa cells labeling Histone H3 (mono methyl K4) using ab195391 at a 1/200 dilution.
Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the ab195391 (left) diluted 1/200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The right panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown below.
