Anti-Histone H3 (mono methyl K27) antibody (ab195492)
Key features and details
- Rabbit polyclonal to Histone H3 (mono methyl K27)
- Suitable for: Dot blot, ICC/IF, ChIP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (mono methyl K27) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (mono methyl K27) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, ICC/IF, ChIP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Arabidopsis thaliana
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Immunogen
Synthetic peptide corresponding to Histone H3 (methyl K27) conjugated to keyhole limpet haemocyanin. Peptide surrounding K27.
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Positive control
- Human HeLa and Human osteosarcoma (U2OS) cells. Histone extracts from HeLa cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-Histone H3 (mono methyl K27) antibody (ab195492)
ChIP analyis using Human HeLa cells, labeling Histone H3 (mono methyl K27) with ab195492 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the active gene GAPDH used as a negative and a positive control target, respectively. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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Dot Blot - Anti-Histone H3 (mono methyl K27) antibody (ab195492)Dot Blot analysis of peptides containing modified and unmodified sequences of histone H3 and H4, labeling Histone H3 (mono methyl K27) with ab195492 at 1/20000 dilution. 0.2-100 pmol of the peptide containing the respective histone modification were spotted onto the membrane.
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Western blot - Anti-Histone H3 (mono methyl K27) antibody (ab195492)Anti-Histone H3 (mono methyl K27) antibody (ab195492) at 1/1000 dilution (in TBS-Tween containing 5% skimmed milk) + Histone extracts from HeLa cells at 15 µg
Predicted band size: 15 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K27) antibody (ab195492)Immunofluorescent analysis of Human osteosarcoma (U2OS) cells labeling Histone H3 (mono methyl K27) with ab195492 at 1/1000 dilution in blocking solution followed by A (top row) an anti-rabbit antibody conjugated to Alexa568 (left) or with DAPI (right), which specifically labels DNA. Cells were fixed with 4% formaldehyde for 20 minutes and blocked with PBS/TX-100 containing 5% normal goat serum.
Rows 2-5: staining of the cells with the ab195492 after incubation of the antibody with 2 ng/μl blocking peptide containing the unmodified (row 2) and the mono- (row 3), di- (row 4) and trimethylated H3K27 (row 5), respectively.
